VOLUME IV. Special Techniques for the Enzymologist

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Section I. Techniques for Characterization of Proteins (Procedures and Interpretations)

1. Electrophoresis; Macro and Micro.
2. Ultracentrifugation and Related Techniques (Diffusion, Viscosity) for Molecular Size and Shape.
3. Infra-red Spectrophotometry.
4. X-ray Diffraction.
5. Light Scattering Measurements.
6. Flow Birefringence.
7. Fluorescence Polarization and Other Fluorescence Techniques.
8. The Solubility Method for Protein Purity.
9. Determination of Amino Acid Sequence in Proteins.
10. Determination of Essential Groups for Enzyme Activity.

Section II. Techniques for Metabolic Studies

1. Measurement of Rapid Reaction Rates; Techniques and Applications, Including Determination of Spectra of Cytochromes and Other Electron Carriers in Respiring Cells.
2. Use of Artificial Electron Acceptors in the Study of Dehydrogenases.
3. Use of Percolation Technique for the Study of the Metabolism of Soil Microorganisms.
4. Methods for Study of the Hill Reaction.
5. Methods for Measurement of Nitrogen Fixation.
6. Cytochemistry.

Section III. Techniques for Isotope Studies

1. The Measurement of Isotopes.
2. The Synthesis and Degradation of Labeled Compounds (Including Application to Metabolic Studies): Monosaccharides and Polysaccharides; Citric Acid Cycle Intermediates; Glycolic, Glyoxylic and Oxalic Acids; Purines and Pyrimidines; Porphyrins; Amino Acids and Proteins; Steroids; Methylated Compounds and Derivatives; Sulfur Compounds; Fatty Acids; Phospholipids; Coenzymes; Iodinated Compounds; Intermediates of Photosynthesis; O18-Labeled Phosphorus Compounds.