VOLUME 583. Enzymology at the Membrane Interface: Interfacial Enzymology and Protein-Membrane Binding

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"Volume 583 of Methods in Enzymology is focused on interfacial enzymes that act on lipid substrates. Methods for detecting and quantifying the binding of proteins and enzymes to membranes in vitro are covered in Chapters 1, 9, 10, and 11. Methods for detection of interfacial binding of proteins and enzymes to membranes in living cells are covered in Chapters 2, 4, 6, 13, and 15. Some interfacial enzymes form protein–protein complexes, and this is described in Chapters 8 and 14. Studies to evaluate the substrate specificity of interfacial enzymes on natural members are covered in Chapter 5. Novel spectroscopic studies for providing structural insight into protein–membrane binding are provided in Chapters 7, 9, 11, and 12. Methods for production of recombinant interfacial enzymes are given in Chapters 3 and 6."