VOLUME 573. Enzymes of Epigenetics, Part A
Available here.
"In Volume 1, Chapters 1 through 5 cover Chromatin Structure and Histones. This includes strategies for in vitro chromatin assembly (Chapter 1) and the assembly of protein–chromatin complexes (Chapter 2) for biochemical, biophysical, and structural studies, preparation of recombinant centromeric nucleosomes with and without nonhistone centromere proteins (Chapter 3), functional characterization of histone deposition by histone chaperones (Chapter 4), and methods to study nucleosome sliding by ATP-dependent chromatin remodeling enzymes (Chapter 5). In Volume 1, Chapters 6 through 13 cover Posttranslational Histone Modification Enzymes and Complexes. This includes in vitro activity assays for MYST histone acetyltransferases and adaptation for high-throughput inhibitor screening (Chapter 6), and the preparation, biochemical analysis, and structure determination of the classical (Chapter 7) and sirtuin (Chapter 8) histone lysine deacetylases, SET domain histone methyltransferases (Chapter 9), LSD1/KDM1A (Chapter 10), and JmjC (Chapter 12). Also included are chapters on LSD1 histone demethylase assays and inhibition (Chapter 11) and preparation and analysis of native chromatin-modifying complexes (Chapter 13). In Volume 1, Chapters 14 and 15 cover Histone Modification Binders and include the preparation, biochemical analysis, and structure determination of the acetyl-lysine reader bromodomains (Chapter 14) and the readers of the methyllysine mark (Chapter 15). In Volume 1, Chapters 16 through 20 cover DNA Modifications and Nucleic Acid Regulators. This includes quantification of oxidized 5-methylcytosine bases and TET enzyme activity (Chapter 16), characterization of how DNA modifications affect DNA binding by C2H2 zinc finger proteins (Chapter 17), regulation of chromosome ends by the riboprotein telomerase complex (Chapter 18), detection and analysis of long noncoding RNAs (Chapter 19), and identification of centromeric RNAs involved in histone dynamics in vivo (Chapter 20)."
