apimba - User contributions [en]

VOLUME 620. New Approaches for Flavin Catalysis

2026-07-06T01:19:49Z

Maintenance script: Fix broken external PDF/book links (pdfdrive, epdf, dead PDFs)

I could not find this volume online except for commercial sites for hundreds of dollars. If someone funds my research then I will be happy to purchase it, until then, if you have a copy email it to [mailto:jstewart@apimba.org me]!

An open-access review on flavin biocatalysts is [https://pmc.ncbi.nlm.nih.gov/articles/PMC6605781/ available at PMC].

VOLUME 606. Radical SAM Enzymes

2026-07-06T01:19:48Z

Maintenance script: Fix broken external PDF/book links (pdfdrive, epdf, dead PDFs)

I could not find this volume online except for commercial sites for hundreds of dollars. If someone funds my research then I will be happy to purchase it, until then, if you have a copy email it to [mailto:jstewart@apimba.org me]!

An open-access review on radical SAM enzymes is [https://pmc.ncbi.nlm.nih.gov/articles/PMC8326336/ available at PMC].

VOLUME XVII. Metabolism of Amino Acids and Amines (Parts A and B)

2026-07-06T01:19:24Z

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<p>Part A is Available at [https://openlibrary.org/search?q=Methods%20in%20Enzymology%20Volume%20XVII%20Metabolism%20of%20Amino%20Acids%20and%20Amines%20%28Parts%20A%20and%20B%29 Open Library] or [https://archive.org/search?q=Methods%20in%20Enzymology%20Volume%20XVII%20Metabolism%20of%20Amino%20Acids%20and%20Amines%20%28Parts%20A%20and%20B%29 Internet Archive].</p>
<p>Part B is available [https://af.u1lib.org/book/2337278/73da16 here].</p>

"The first section of Volume XVIIA includes an extensive description of various microbiological and genetic techniques that have become major tools in the study of amino acid metabolism in recent years. Since there has been a paucity of manuals giving specific details of these methods, we hope that the articles published here will be of considerable value. The remainder of the material is arranged in sections which are in a roughly alphabetical order: alanine to guanido compounds in XVIIA and the remaining amino acids and amine metabolism sections in XVIIB."

VOLUME 645. Extracellular vesicles

2026-07-06T01:19:24Z

Maintenance script: Fix broken external PDF/book links (pdfdrive, epdf, dead PDFs)

I could not find this volume online except for commercial sites for hundreds of dollars. If someone funds my research then I will be happy to purchase it, until then, if you have a copy email it to [mailto:jstewart@apimba.org me]!

A similar book from the Methods in Molecular Biology series is Available at [https://openlibrary.org/search?q=Methods%20in%20Enzymology%20Volume%20645%20Extracellular%20vesicles Open Library] or [https://archive.org/search?q=Methods%20in%20Enzymology%20Volume%20645%20Extracellular%20vesicles Internet Archive].

VOLUME 644. Enzyme Engineering and Evolution: Specific Enzyme Applications

2026-07-06T01:19:23Z

Maintenance script: Fix broken external PDF/book links (pdfdrive, epdf, dead PDFs)

I could not find this volume online except for commercial sites for hundreds of dollars. If someone funds my research then I will be happy to purchase it, until then, if you have a copy email it to [mailto:jstewart@apimba.org me]!

A related volume from the Methods in Molecular Biology series is Available at [https://openlibrary.org/search?q=Methods%20in%20Enzymology%20Volume%20644%20Enzyme%20Engineering%20and%20Evolution%3A%20Specific%20Enzyme%20Applications Open Library] or [https://archive.org/search?q=Methods%20in%20Enzymology%20Volume%20644%20Enzyme%20Engineering%20and%20Evolution%3A%20Specific%20Enzyme%20Applications Internet Archive].

VOLUME 637. Retinoid Signaling Pathways

2026-07-06T01:19:22Z

Maintenance script: Fix broken external PDF/book links (pdfdrive, epdf, dead PDFs)

I could not find this volume online except for commercial sites for hundreds of dollars. If someone funds my research then I will be happy to purchase it, until then, if you have a copy email it to [mailto:jstewart@apimba.org me]!

A related volume from the Methods in Molecular Biology series is Available at [https://openlibrary.org/search?q=Methods%20in%20Enzymology%20Volume%20637%20Retinoid%20Signaling%20Pathways Open Library] or [https://archive.org/search?q=Methods%20in%20Enzymology%20Volume%20637%20Retinoid%20Signaling%20Pathways Internet Archive].

VOLUME 627. Enzymatic Polymerizations

2026-07-06T01:19:21Z

Maintenance script: Fix broken external PDF/book links (pdfdrive, epdf, dead PDFs)

I could not find this volume online except for commercial sites for hundreds of dollars. If someone funds my research then I will be happy to purchase it, until then, if you have a copy email it to [mailto:jstewart@apimba.org me]!

A related book is Available at [https://openlibrary.org/search?q=Methods%20in%20Enzymology%20Volume%20627%20Enzymatic%20Polymerizations Open Library] or [https://archive.org/search?q=Methods%20in%20Enzymology%20Volume%20627%20Enzymatic%20Polymerizations Internet Archive].

VOLUME 626. Post-translational Modifications That Modulate Enzyme Activity

2026-07-06T01:19:21Z

Maintenance script: Fix broken external PDF/book links (pdfdrive, epdf, dead PDFs)

I could not find this volume online except for commercial sites for hundreds of dollars. If someone funds my research then I will be happy to purchase it, until then, if you have a copy email it to [mailto:jstewart@apimba.org me]!

A similar volume from the Methods in Molecular Biology series is Available at [https://openlibrary.org/search?q=Methods%20in%20Enzymology%20Volume%20626%20Post-translational%20Modifications%20That%20Modulate%20Enzyme%20Activity Open Library] or [https://archive.org/search?q=Methods%20in%20Enzymology%20Volume%20626%20Post-translational%20Modifications%20That%20Modulate%20Enzyme%20Activity Internet Archive].

VOLUME 625. DNA Sensors and Inflammasomes

2026-07-06T01:19:20Z

Maintenance script: Fix broken external PDF/book links (pdfdrive, epdf, dead PDFs)

I could not find this volume online except for commercial sites for hundreds of dollars. If someone funds my research then I will be happy to purchase it, until then, if you have a copy email it to [mailto:jstewart@apimba.org me]!

A book reviewing inflammasomes is Available at [https://openlibrary.org/search?q=Methods%20in%20Enzymology%20Volume%20625%20DNA%20Sensors%20and%20Inflammasomes Open Library] or [https://archive.org/search?q=Methods%20in%20Enzymology%20Volume%20625%20DNA%20Sensors%20and%20Inflammasomes Internet Archive].

VOLUME 612. High-Density Sequencing Applications in Microbial Molecular Genetics

2026-07-06T01:19:19Z

Maintenance script: Fix broken external PDF/book links (pdfdrive, epdf, dead PDFs)

I could not find this volume online except for commercial sites for hundreds of dollars. If someone funds my research then I will be happy to purchase it, until then, if you have a copy email it to [mailto:jstewart@apimba.org me]!

A volume about sequencing from the Methods in Molecular Biology series is Available at [https://openlibrary.org/search?q=Methods%20in%20Enzymology%20Volume%20612%20High-Density%20Sequencing%20Applications%20in%20Microbial%20Molecular%20Genetics Open Library] or [https://archive.org/search?q=Methods%20in%20Enzymology%20Volume%20612%20High-Density%20Sequencing%20Applications%20in%20Microbial%20Molecular%20Genetics Internet Archive].

VOLUME 593. Cannabinoids and Their Receptors

2026-07-06T01:19:18Z

Maintenance script: Fix broken external PDF/book links (pdfdrive, epdf, dead PDFs)

I could not find this volume online except for commercial sites for hundreds of dollars. If someone funds my research then I will be happy to purchase it, until then, if you have a copy email it to [mailto:jstewart@apimba.org me]!

A related book is Available at [https://openlibrary.org/search?q=Methods%20in%20Enzymology%20Volume%20593%20Cannabinoids%20and%20Their%20Receptors Open Library] or [https://archive.org/search?q=Methods%20in%20Enzymology%20Volume%20593%20Cannabinoids%20and%20Their%20Receptors Internet Archive].

VOLUME XXXVIII. Hormone Action (Part C: Cyclic Nucleotides)

2026-07-06T01:18:32Z

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Available at [https://openlibrary.org/search?q=Methods%20in%20Enzymology%20Volume%20XXXVIII%20Hormone%20Action%20%28Part%20C%3A%20Cyclic%20Nucleotides%29 Open Library] or [https://archive.org/search?q=Methods%20in%20Enzymology%20Volume%20XXXVIII%20Hormone%20Action%20%28Part%20C%3A%20Cyclic%20Nucleotides%29 Internet Archive].

"This volume contains a broad selection of techniques that should be useful to those interested in studying the cyclic nucleotide content of intact cells and the biosynthesis, degradation, and action of cyclic nucleotides in cell-free systems. Techniques for purifying and assaying a single enzyme or nucleotide will be described in more than one article. This is largely by editorial design, not to compile a complete collection of methods, but to offer the investigator a choice of useful techniques. All of them will be reliable when properly used, but none of them will be, for all laboratories, either easier or more reliable to use than any of the others. In some cases (as, for example, with adenylate cyclase from mammalian sources) lability or lack of extensive purification of an enzyme has contributed to technical problems that differ from tissue to tissue. In other cases (as, for example, with cyclic nucleotide phosphodiesterase) certain properties of an enzyme will be found to be distinctly different from tissue to tissue or among multiple forms of the enzyme from a single tissue."

VOLUME XXXVII. Hormone Action (Part B: Peptide Hormones)

2026-07-06T01:18:31Z

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Available at [https://openlibrary.org/search?q=Methods%20in%20Enzymology%20Volume%20XXXVII%20Hormone%20Action%20%28Part%20B%3A%20Peptide%20Hormones%29 Open Library] or [https://archive.org/search?q=Methods%20in%20Enzymology%20Volume%20XXXVII%20Hormone%20Action%20%28Part%20B%3A%20Peptide%20Hormones%29 Internet Archive].

"This volume deals with methods for assaying protein hormones and for monitoring their interaction with specific plasma membrane receptors. Descriptions of biologic systems that have been devised to monitor the biochemical effects of protein hormones are presented. In addition, purification and synthesis of peptide hormones are included since the chemical purity of the hormonal effector is of ultimate importance."

VOLUME XXXVI. Hormone Action (Part A: Steroid Hormones)

2026-07-06T01:18:31Z

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Available at [https://openlibrary.org/search?q=Methods%20in%20Enzymology%20Volume%20XXXVI%20Hormone%20Action%20%28Part%20A%3A%20Steroid%20Hormones%29 Open Library] or [https://archive.org/search?q=Methods%20in%20Enzymology%20Volume%20XXXVI%20Hormone%20Action%20%28Part%20A%3A%20Steroid%20Hormones%29 Internet Archive].

"This volume contains a compilation of techniques, and as such is intended to provide a methodological reference for studies of steroid hormone action. Thyroid hormones are also included in this volume because of their apparent similarities in regard to molecular mechanism of action. Considerations are provided concerning the manner in which hormones circulate in the blood stream and are eventually sequestered in target cells. This leads to description of their interactions with cytoplasmic and nuclear receptors as well as presentation of techniques for purification of the steroid hormone receptor molecules. Methods dealing with the biochemical and biological processes stimulated by steroid hormones are included in addition to methods for assessing hormone metabolism. Although a good deal of work and interest in this field has been at the level of gene transcription and nucleic acid synthesis, such methods have been and will continue to be dealt with in other volumes of this series."

VOLUME XXXV. Lipids (Part B)

2026-07-06T01:18:30Z

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Available at [https://openlibrary.org/search?q=Methods%20in%20Enzymology%20Volume%20XXXV%20Lipids%20%28Part%20B%29 Open Library] or [https://archive.org/search?q=Methods%20in%20Enzymology%20Volume%20XXXV%20Lipids%20%28Part%20B%29 Internet Archive].

"This volume covers recent developments in the enzymology of lipids. The enzymes included are in general those that have been purified to homogeneity or that have been purified highly."

VOLUME XXXIX. Hormone Action (Part D: Isolated Cells, Tissues, and Organ Systems)

2026-07-06T01:18:30Z

Maintenance script: Fix broken external PDF/book links (pdfdrive, epdf, dead PDFs)

Available at [https://openlibrary.org/search?q=Methods%20in%20Enzymology%20Volume%20XXXIX%20Hormone%20Action%20%28Part%20D%3A%20Isolated%20Cells%2C%20Tissues%2C%20and%20Organ%20Systems%29 Open Library] or [https://archive.org/search?q=Methods%20in%20Enzymology%20Volume%20XXXIX%20Hormone%20Action%20%28Part%20D%3A%20Isolated%20Cells%2C%20Tissues%2C%20and%20Organ%20Systems%29 Internet Archive].

"Our aim in compiling this volume was to provide a source of descriptions and critical evaluations of currently useful intact cell systems and general techniques applicable to the study of hormone action on biochemical and biophysical parameters of intact cells. Many of these systems and techniques will be useful for studying the actions of a variety of hormones, neurotransmitters, and pharmacological agents. Others will be suitable primarily for investigators whose interests arc confined to actions of a single hormone or class of hormones."

VOLUME XXXIV. Affinity Techniques (Enzyme Purification: Part B)

2026-07-06T01:18:29Z

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Available at [https://openlibrary.org/search?q=Methods%20in%20Enzymology%20Volume%20XXXIV%20Affinity%20Techniques%20%28Enzyme%20Purification%3A%20Part%20B%29 Open Library] or [https://archive.org/search?q=Methods%20in%20Enzymology%20Volume%20XXXIV%20Affinity%20Techniques%20%28Enzyme%20Purification%3A%20Part%20B%29 Internet Archive].

"Although our approach and concentration have been oriented toward enzyme purification, affinity methods are presented for such diverse systems as cells, antibodies, and specifically modified proteins. The material on antibody serves mainly as a guide to the enzymologist since methods specific to working with these species of protein have been examined in great detail elsewhere."

VOLUME XXXII. Biomembranes (Part B)

2026-07-06T01:18:28Z

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Available at [https://openlibrary.org/search?q=Methods%20in%20Enzymology%20Volume%20XXXII%20Biomembranes%20%28Part%20B%29 Open Library] or [https://archive.org/search?q=Methods%20in%20Enzymology%20Volume%20XXXII%20Biomembranes%20%28Part%20B%29 Internet Archive].

VOLUME XXXI. Biomembranes (Part A)

2026-07-06T01:18:27Z

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Available at [https://openlibrary.org/search?q=Methods%20in%20Enzymology%20Volume%20XXXI%20Biomembranes%20%28Part%20A%29 Open Library] or [https://archive.org/search?q=Methods%20in%20Enzymology%20Volume%20XXXI%20Biomembranes%20%28Part%20A%29 Internet Archive].

"Several volumes on biomembranes have been planned. The first two (XXXI and XXXII) deal with techniques in the isolation and characterization of cells, organelles, membranes, and their components. A third volume will be devoted to electron transport and oxidative phosphorylation. It will update the very useful "Oxidation and Phosphorylation," Volume X which was edited by R. W. Estabrook and M. E. Pullman. Yet another volume will concentrate on methods for the study of properties of membranes, their architecture and function, including the study of biological transport and membrane receptors."

VOLUME XXX. Nucleic Acids and Protein Synthesis (Part F)

2026-07-06T01:18:27Z

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Available at [https://openlibrary.org/search?q=Methods%20in%20Enzymology%20Volume%20XXX%20Nucleic%20Acids%20and%20Protein%20Synthesis%20%28Part%20F%29 Open Library] or [https://archive.org/search?q=Methods%20in%20Enzymology%20Volume%20XXX%20Nucleic%20Acids%20and%20Protein%20Synthesis%20%28Part%20F%29 Internet Archive].

"This volume deals with the various prokaryotic and eukaryotic systems that can carry out protein synthesis and/or intermediary reactions involved in this process. A portion of the volume is devoted to some new and improved methods for examining the roles of initiation, elongation, and termination factors. A fairly extensive section describes the preparation, physical and biological characterization, and the protein compositional analysis of ribosomes and their corresponding subunits; assays for individual reactions catalyzed by these particles are also included. The isolation of several messenger RNA's and the preparation of a number of biological systems capable of de novo synthesis of complete, identifiable proteins in vitro, particularly from eukaryotic cells, are also described."

VOLUME XXVIII. Complex Carbohydrates (Part B)

2026-07-06T01:18:26Z

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Available at [https://openlibrary.org/search?q=Methods%20in%20Enzymology%20Volume%20XXVIII%20Complex%20Carbohydrates%20%28Part%20B%29 Open Library] or [https://archive.org/search?q=Methods%20in%20Enzymology%20Volume%20XXVIII%20Complex%20Carbohydrates%20%28Part%20B%29 Internet Archive].

"This volume covers material on complex carbohydrates that has appeared in the literature since the publication in 1966 of Volume VIII on this subject. It has the same subdivisions as Volume VIII plus a section on the purification and properties of carbohydrate-binding proteins."

VOLUME XXVII. Enzyme Structure (Part D)

2026-07-06T01:18:26Z

Maintenance script: Fix broken external PDF/book links (pdfdrive, epdf, dead PDFs)

Available at [https://openlibrary.org/search?q=Methods%20in%20Enzymology%20Volume%20XXVII%20Enzyme%20Structure%20%28Part%20D%29 Open Library] or [https://archive.org/search?q=Methods%20in%20Enzymology%20Volume%20XXVII%20Enzyme%20Structure%20%28Part%20D%29 Internet Archive].

"This is the second of two volumes of "Enzyme Structure" devoted to physical methods. (Part C, Volume 26 of "Methods in Enzymology," appeared recently.) Although coverage of the various techniques is not exhaustive, it is hoped that the intent of presenting a broad coverage of currently available methods has been reasonably fulfilled."

"These volumes present not only techniques that are currently widely available but some which are only beginning to make an impact and some for which no commercial standard equipment is as yet available. In the latter cases, an attempt has been made to guide the reader in assembling his own equipment from individual components and to help him find the necessary information in the research literature."

"In the coverage of physical techniques, we have departed somewhat in scope from the traditional format of the series. Since, at the termination of an experiment, physical techniques frequently require much more interpretation than do organic ones, we consider that brief sections on the theoretical principles involved are highly desirable as are sections on theoretical and mathematical approaches to data evaluation and on assumptions and, consequently, limitations involved in the applications of the various methods."

"The division of the material between the two parts is arbitrary. Thus, there is a considerable amount of overlap between general categories, and, at times, the descriptions of closely related techniques are found divided between Parts C and D. We do not believe, however, that this should hinder the reader in his use of these volumes for, in every case, each chapter is a completely self-contained unit."

VOLUME XXVI. Enzyme Structure (Part C)

2026-07-06T01:18:26Z

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Available at [https://openlibrary.org/search?q=Methods%20in%20Enzymology%20Volume%20XXVI%20Enzyme%20Structure%20%28Part%20C%29 Open Library] or [https://archive.org/search?q=Methods%20in%20Enzymology%20Volume%20XXVI%20Enzyme%20Structure%20%28Part%20C%29 Internet Archive].

""Enzyme Structure," Parts A and B, the eleventh and twenty-fifth volumes of this series, were concerned mostly with chemical techniques. The section on physical methods in Part A was highly restricted. At present, two supplementary volumes dealing in detail with physical methods have been prepared, this volume and Volume 27, "Enzyme Structure," Part D, which is now in press. It is hoped that these will give a broad coverage of techniques currently available for the study of enzyme conformation and interactions."

"In 1957, physical methods were first covered in Volume 4 of this series. The current volumes update and vastly amplify the original coverage. In these volumes an attempt has been made to present not only techniques which are currently widely available, but some which are only beginning to make an impact and some for which no commercial standard equipment is as yet available. In the latter cases, an attempt has been made to guide the reader in assembling his own equipment from individual components and to help him find the necessary information in the research literature."

"In the coverage of physical techniques, we have departed somewhat in scope from the traditional format of the series. Since, at the termination of an experiment, physical techniques frequently require much more interpretation than do organic techniques, we consider that brief sections on the theoretical principles involved are highly desirable as are sections on theoretical and mathematical approaches to data evaluation and on assumptions and, consequently, limitations involved in the applications of the various methods."

"The division of the material between the two parts is arbitrary. Thus, there will be a considerable amount of overlap between general categories, and, at times, the descriptions of closely related techniques will be found divided between Parts C and D. We do not believe, however, that this will hinder the reader in his use of these volumes for, in every case, each chapter is a completely self-contained unit."

VOLUME XXV. Enzyme Structure (Part B)

2026-07-06T01:18:25Z

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Available at [https://openlibrary.org/search?q=Methods%20in%20Enzymology%20Volume%20XXV%20Enzyme%20Structure%20%28Part%20B%29 Open Library] or [https://archive.org/search?q=Methods%20in%20Enzymology%20Volume%20XXV%20Enzyme%20Structure%20%28Part%20B%29 Internet Archive].

""Enzyme Structure," the eleventh volume in this series, first appeared in 1967. Although the objectives sought in its publication have largely been realized, it had been apparent almost from the beginning that to maintain its usefulness a periodic revision and updating of the text would be necessary. The original volume, moreover, contained some notable omissions. In particular, the scope of the section on conformational changes was restricted deliberately in the expectation that ultimately another volume would be published in which physical methods generally would be covered in a more comprehensive fashion."

"This volume should be regarded as the first of three supplements to Volume XI. It is designed to update the first nine sections of Volume XI and for that reason retains the organization of that volume. In addition to a number of new procedures not previously covered, it includes revised and updated versions of most of the articles from Volume XI which experience has shown to be those most frequently referred to in the literature. The last section on methods suitable for the investigation of conformational changes will be substantially enlarged upon in two additional volumes presently in preparation."

VOLUME XXIX. Nucleic Acids and Protein Synthesis (Part E)

2026-07-06T01:18:25Z

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Available at [https://openlibrary.org/search?q=Methods%20in%20Enzymology%20Volume%20XXIX%20Nucleic%20Acids%20and%20Protein%20Synthesis%20%28Part%20E%29 Open Library] or [https://archive.org/search?q=Methods%20in%20Enzymology%20Volume%20XXIX%20Nucleic%20Acids%20and%20Protein%20Synthesis%20%28Part%20E%29 Internet Archive].

"The introduction of two additional volumes dealing with nucleic acids and protein synthesis (Volume XXIX, Part E and Volume XXX, Part F) attests to the remarkable progress that continues to be made in these fields of research."

"In this volume detailed descriptions for the isolation, purification, and properties of DNA polymerases from a variety of prokaryotic and eukaryotic organisms are given. In addition, a description of those proteins that may participate in the replication process in an ancillary manner is presented. Techniques with which the activities of DNA polymerases may be assessed under conditions in vivo by virtue of the employment of cells with modified permeability properties are also included. The isolation and characterization of those DNA polymerases from animal cells and viruses capable of transcribing RNA are described in detail."

VOLUME XXIV. Photosynthesis and Nitrogen Fixation (Part B)

2026-07-06T01:18:24Z

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Available at [https://openlibrary.org/search?q=Methods%20in%20Enzymology%20Volume%20XXIV%20Photosynthesis%20and%20Nitrogen%20Fixation%20%28Part%20B%29 Open Library] or [https://archive.org/search?q=Methods%20in%20Enzymology%20Volume%20XXIV%20Photosynthesis%20and%20Nitrogen%20Fixation%20%28Part%20B%29 Internet Archive].

"Volume XXIV will cover methodology both chemical and physical, buffers and inhibitors, synthesizing capabilities of the photochemical systems, and a short section devoted to the analytical techniques and isolation of components of nitrogen fixation. Where appropriate, reference is provided to articles published previously in this series."

VOLUME XXIII. Photosynthesis and Nitrogen (Part A)

2026-07-06T01:18:23Z

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Available at [https://openlibrary.org/search?q=Methods%20in%20Enzymology%20Volume%20XXIII%20Photosynthesis%20and%20Nitrogen%20%28Part%20A%29 Open Library] or [https://archive.org/search?q=Methods%20in%20Enzymology%20Volume%20XXIII%20Photosynthesis%20and%20Nitrogen%20%28Part%20A%29 Internet Archive].

"The presentations in this volume consider isolation and culture techniques of algae, bacteria, and diatoms; plant tissue culture; the preparation and properties of mutants; cellular and subcellular preparations from algae, bacteria, and plants; and the purification and properties of components of the photosynthetic systems. Volume XXIV will cover methodology both chemical and physical, buffers and inhibitors, synthesizing capabilities of the photochemical systems, and a short section devoted to the analytical techniques and isolation of components of nitrogen fixation. Where appropriate, reference is provided to articles published previously in this series."

VOLUME XXII. Enzyme Purification and Related Techniques

2026-07-06T01:18:22Z

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Available at [https://openlibrary.org/search?q=Methods%20in%20Enzymology%20Volume%20XXII%20Enzyme%20Purification%20and%20Related%20Techniques Open Library] or [https://archive.org/search?q=Methods%20in%20Enzymology%20Volume%20XXII%20Enzyme%20Purification%20and%20Related%20Techniques Internet Archive].

"Aside from methods related directly to purification, this volume includes a description of peripheral techniques of value in enzyme preparation. For example, much space has been allowed for the methodology of obtaining cells which must be grown in the laboratory, and emphasis has been placed on the isolation of specialized organelles. Many useful general methods which have been included in previous volumes of this series are listed in the section to which they apply. Such a list is not only particularly useful as a source of direction for isolating specific organelles but also explains why a separate article was not included for the single, most common means of enzyme purification, salting out. The latter was so well recorded in a previous volume that reference to the article should suffice."

VOLUME XXI. Nucleic Acids (Part D)

2026-07-06T01:18:21Z

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Available at [https://openlibrary.org/search?q=Methods%20in%20Enzymology%20Volume%20XXI%20Nucleic%20Acids%20%28Part%20D%29 Open Library] or [https://archive.org/search?q=Methods%20in%20Enzymology%20Volume%20XXI%20Nucleic%20Acids%20%28Part%20D%29 Internet Archive].

VOLUME XX. Nucleic Acids and Protein Synthesis (Part C)

2026-07-06T01:18:21Z

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Available at [https://openlibrary.org/search?q=Methods%20in%20Enzymology%20Volume%20XX%20Nucleic%20Acids%20and%20Protein%20Synthesis%20%28Part%20C%29 Open Library] or [https://archive.org/search?q=Methods%20in%20Enzymology%20Volume%20XX%20Nucleic%20Acids%20and%20Protein%20Synthesis%20%28Part%20C%29 Internet Archive].

"Since the publication of the previous two volumes of "Methods in Enzymology" dealing with nucleic acids, this field of research has seen continued and rapid development. In order to maintain a comprehensive coverage of pertinent methodology for workers in the biological sciences, this compilation is extended in the form of two additional volumes: Volume 20, "Nucleic Acids and Protein Synthesis," Part C, and Volume 21, "Nucleic Acids," Part D. Part C deals with areas of protein synthesis in which nucleic acids play an integral role. Portions of this volume are devoted to the preparation and the chemical and biological properties of tRNA; to the initiation, elongation, and termination of peptide chains in translation; to the structure and function of ribosomes; and to the synthesis of characterizable proteins in vitro. We dedicate this volume to the late Dr. Richard Schweet who pioneered in many of these findings."

VOLUME XV. Steroids and Terpenoids

2026-07-06T01:18:20Z

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Available at [https://openlibrary.org/search?q=Methods%20in%20Enzymology%20Volume%20XV%20Steroids%20and%20Terpenoids Open Library] or [https://archive.org/search?q=Methods%20in%20Enzymology%20Volume%20XV%20Steroids%20and%20Terpenoids Internet Archive].

"This resulting volume, devoted to methodology in the steroid and terpenoid fields, is divided into four main sections that deal, respectively, with special analytical methods, methods of synthesis of labeled substrates, enzyme preparation methods, and steroid-binding protein methods as exemplified by corticosteroid binding globulin (C.B.G.). As in the other volumes of this publication, the aim has been to include details of new procedures or improvements in existing methods that have become available since the last contributions in the area of steroid biochemistry were made to the treatise. Thus, in the analytical section, thin-layer chromatography and a number of applications of gas-liquid chromatography receive particular attention, and, with regard to the latter, methods for the isolation of suitably purified extracts for analysis by the highly sensitive electron-capture technique are emphasized."

VOLUME XLVIII. Enzyme Structure (Part F)

2026-07-06T01:18:20Z

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Available at [https://openlibrary.org/search?q=Methods%20in%20Enzymology%20Volume%20XLVIII%20Enzyme%20Structure%20%28Part%20F%29 Open Library] or [https://archive.org/search?q=Methods%20in%20Enzymology%20Volume%20XLVIII%20Enzyme%20Structure%20%28Part%20F%29 Internet Archive].

VOLUME XLVII. Enzyme Structure (Part E)

2026-07-06T01:18:19Z

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Available at [https://openlibrary.org/search?q=Methods%20in%20Enzymology%20Volume%20XLVII%20Enzyme%20Structure%20%28Part%20E%29 Open Library] or [https://archive.org/search?q=Methods%20in%20Enzymology%20Volume%20XLVII%20Enzyme%20Structure%20%28Part%20E%29 Internet Archive].

VOLUME XLVI. Affinity Labeling

2026-07-06T01:18:19Z

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Available at [https://openlibrary.org/search?q=Methods%20in%20Enzymology%20Volume%20XLVI%20Affinity%20Labeling Open Library] or [https://archive.org/search?q=Methods%20in%20Enzymology%20Volume%20XLVI%20Affinity%20Labeling Internet Archive].

"In view of the rapidly expanding record of successful examples of affinity labeling, a number of guidelines are presented in the first section of this volume, that on General Methodology, which include the critical basis for design as well as a number of synthetic procedures of wide applicability. The specific instances that are analyzed in the subsequent sections represent both the triumphs and shortcomings of the technique of affinity labeling in its various guises."

VOLUME XLIX. Enzyme Structure (Part G)

2026-07-06T01:18:18Z

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Available at [https://openlibrary.org/search?q=Methods%20in%20Enzymology%20Volume%20XLIX%20Enzyme%20Structure%20%28Part%20G%29 Open Library] or [https://archive.org/search?q=Methods%20in%20Enzymology%20Volume%20XLIX%20Enzyme%20Structure%20%28Part%20G%29 Internet Archive].

VOLUME XLIV. Immobilized Enzymes

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Available at [https://openlibrary.org/search?q=Methods%20in%20Enzymology%20Volume%20XLIV%20Immobilized%20Enzymes Open Library] or [https://archive.org/search?q=Methods%20in%20Enzymology%20Volume%20XLIV%20Immobilized%20Enzymes Internet Archive].

"As a result, this volume does differ somewhat from the usual format. Many sections begin with a general methodological review, and are followed by specific examples. In the introductory paper, as a further guide, the background of each section is given."

"The term "immobilized enzymes" has been used following a recommendation made at the 1973 Enzyme Engineering Conference in Henniker, New Hampshire. However, other terms, such as matrix-bound" and "insolubilized," to refer to the same type of preparation and "support," "matrix," and "carrier" are used indiscriminately."

VOLUME XLII. Carbohydrate Metabolism (Part C)

2026-07-06T01:18:17Z

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Available at [https://openlibrary.org/search?q=Methods%20in%20Enzymology%20Volume%20XLII%20Carbohydrate%20Metabolism%20%28Part%20C%29 Open Library] or [https://archive.org/search?q=Methods%20in%20Enzymology%20Volume%20XLII%20Carbohydrate%20Metabolism%20%28Part%20C%29 Internet Archive].

VOLUME XLI. Carbohydrate Metabolism (Part B)

2026-07-06T01:18:16Z

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Available at [https://openlibrary.org/search?q=Methods%20in%20Enzymology%20Volume%20XLI%20Carbohydrate%20Metabolism%20%28Part%20B%29 Open Library] or [https://archive.org/search?q=Methods%20in%20Enzymology%20Volume%20XLI%20Carbohydrate%20Metabolism%20%28Part%20B%29 Internet Archive].

VOLUME XL. Hormone Action (Part E: Nuclear Structure and Function)

2026-07-06T01:18:15Z

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Available at [https://openlibrary.org/search?q=Methods%20in%20Enzymology%20Volume%20XL%20Hormone%20Action%20%28Part%20E%3A%20Nuclear%20Structure%20and%20Function%29 Open Library] or [https://archive.org/search?q=Methods%20in%20Enzymology%20Volume%20XL%20Hormone%20Action%20%28Part%20E%3A%20Nuclear%20Structure%20and%20Function%29 Internet Archive].

"In this volume, we have attempted to collect a series of methodologies which would allow investigators to assess hormone effects on nuclear structure, DNA replication, mitosis, and gene transcription. Techniques for assessing nucleic acid metabolism per se are conspicuously absent from this volume but are thoroughly covered in other volumes of "Methods in Enzymology." In considering gene function and specific gene restriction in eukaryotes, it seems almost certain that these processes will ultimately be related to the composition and location of chromosomal proteins. For this reason, we have emphasized techniques which can be utilized to study chromatin composition, structure, and function. This volume also contains a collection of general techniques and analytical approaches important to those involved in the study of hormone action but which have not been included in the earlier volumes of this series because they did not fall clearly within the previously defined categories."

VOLUME XIX. Proteolytic Enzymes

2026-07-06T01:18:14Z

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Available at [https://openlibrary.org/search?q=Methods%20in%20Enzymology%20Volume%20XIX%20Proteolytic%20Enzymes Open Library] or [https://archive.org/search?q=Methods%20in%20Enzymology%20Volume%20XIX%20Proteolytic%20Enzymes Internet Archive].

"The "Proteolytic Enzymes" like other volumes of "Methods in Enzymology" provides information on the purification and mode of assay of enzymes. It is meant to be of value to the graduate student as well as to the more advanced investigator. The authors have been asked to give an up-to-date synopsis of other knowledge relating to physical properties (molecular weight, electrophoretic behavior, optical rotatory dispersion) and chemical structure (amino acid composition and sequences) as well as of some details on the kinetics of the various reactions catalyzed by the enzymes discussed. We, thus, believe that this volume will fill some of the needs of a primary reference work in this area."

VOLUME XIII. Citric Acid Cycle

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Available at [https://openlibrary.org/search?q=Methods%20in%20Enzymology%20Volume%20XIII%20Citric%20Acid%20Cycle Open Library] or [https://archive.org/search?q=Methods%20in%20Enzymology%20Volume%20XIII%20Citric%20Acid%20Cycle Internet Archive].

"The citric acid cycle represents the terminal stage for the oxidation of the major foodstuffs and energy stores in many organisms. In addition, the cycle plays an important part in the synthesis of many cell constituents from simple precursors. This volume of "Methods in Enzymology" deals with the reactions of the citric acid cycle and with a number of reactions leading to and from the cycle. In deciding what to include in the present volume, boundaries had to be drawn between various areas of metabolism and the citric acid cycle. These boundaries are of necessity somewhat arbitrary. The aspartase reaction is included. On the other hand, enzymes which might have been included here, such as certain transaminases and glutamate dehydrogenase, will be covered in a future volume devoted to amino acid metabolism. A few of the methods which are included should logically have appeared in earlier volumes. These are warranted by the importance of the topic and the time it would take for them to appear in future revisions of recent volumes."

VOLUME XI. Enzyme Structure

2026-07-06T01:18:13Z

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Available at [https://openlibrary.org/search?q=Methods%20in%20Enzymology%20Volume%20XI%20Enzyme%20Structure Open Library] or [https://archive.org/search?q=Methods%20in%20Enzymology%20Volume%20XI%20Enzyme%20Structure Internet Archive].

"The methods selected for coverage have been grouped in the conventional fashion. They progress from amino acid analysis, through chain separation and sequence determination, to procedures for chemical modification and the investigation of conformational changes. This progression would be the one most logically followed in an idealized investigation of structure-function relationships. Inasmuch as the methods of protein chemistry are still in a state of rapid evolution, decisions about what to include and not to include were made not only on the basis of the demonstrated value of a method but from an estimation of its potential as well. For this reason a number of contributions have been included about procedures, which, though employed in a relatively restricted sense, show promise of wider applicability. In this respect, therefore, the volume differs somewhat from its predecessors."

VOLUME X. Oxidation and Phosphorylation

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Available at [https://openlibrary.org/search?q=Methods%20in%20Enzymology%20Volume%20X%20Oxidation%20and%20Phosphorylation Open Library] or [https://archive.org/search?q=Methods%20in%20Enzymology%20Volume%20X%20Oxidation%20and%20Phosphorylation Internet Archive].

"The aim of this volume is to provide not only the specialist and the advanced student but also investigators from other areas of research with a single authoritative source for the vast and often difficult to retrieve and evaluate methodology associated with mitochondrial research."

VOLUME VIII. Complex Carbohydrates

2026-07-06T01:18:12Z

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Available at [https://openlibrary.org/search?q=Methods%20in%20Enzymology%20Volume%20VIII%20Complex%20Carbohydrates Open Library] or [https://archive.org/search?q=Methods%20in%20Enzymology%20Volume%20VIII%20Complex%20Carbohydrates Internet Archive].

"This volume deals with the enzymes involved in the synthesis and degradation of complex saccharides, beginning and ending at the monosaccharide level. The grouping of the enzymes into three sections-- enzymes of sugar activation, of complex saccharide synthesis and of complex saccharide utilization--is based on probable biological function.
The sections on Analytical Methods and Preparation of Substrates could be kept relatively short because of the commercial availability of many compounds and because certain relevant subjects are not only treated in "Carbohydrate Metabolism," a forthcoming volume in the "Methods in Enzymology" series, but also in the "Methods in Carbohydrate Chemistry" series."

VOLUME LX. Nucleic Acids and Protein Synthesis (Part H)

2026-07-06T01:18:12Z

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Available at [https://openlibrary.org/search?q=Methods%20in%20Enzymology%20Volume%20LX%20Nucleic%20Acids%20and%20Protein%20Synthesis%20%28Part%20H%29 Open Library] or [https://archive.org/search?q=Methods%20in%20Enzymology%20Volume%20LX%20Nucleic%20Acids%20and%20Protein%20Synthesis%20%28Part%20H%29 Internet Archive].

VOLUME LVI. Biomembranes (Part G: Bioenergetics)

2026-07-06T01:18:11Z

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Available at [https://openlibrary.org/search?q=Methods%20in%20Enzymology%20Volume%20LVI%20Biomembranes%20%28Part%20G%3A%20Bioenergetics%29 Open Library] or [https://archive.org/search?q=Methods%20in%20Enzymology%20Volume%20LVI%20Biomembranes%20%28Part%20G%3A%20Bioenergetics%29 Internet Archive].

VOLUME LV. Biomembranes (Part F: Bioenergetics)

2026-07-06T01:18:09Z

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Available at [https://openlibrary.org/search?q=Methods%20in%20Enzymology%20Volume%20LV%20Biomembranes%20%28Part%20F%3A%20Bioenergetics%29 Open Library] or [https://archive.org/search?q=Methods%20in%20Enzymology%20Volume%20LV%20Biomembranes%20%28Part%20F%3A%20Bioenergetics%29 Internet Archive].

VOLUME LIX. Nucleic Acids and Protein Synthesis (Part G)

2026-07-06T01:18:08Z

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Available at [https://openlibrary.org/search?q=Methods%20in%20Enzymology%20Volume%20LIX%20Nucleic%20Acids%20and%20Protein%20Synthesis%20%28Part%20G%29 Open Library] or [https://archive.org/search?q=Methods%20in%20Enzymology%20Volume%20LIX%20Nucleic%20Acids%20and%20Protein%20Synthesis%20%28Part%20G%29 Internet Archive].

VOLUME LIV. Biomembranes (Part E: Biological Oxidations)

2026-07-06T01:18:08Z

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Available at [https://openlibrary.org/search?q=Methods%20in%20Enzymology%20Volume%20LIV%20Biomembranes%20%28Part%20E%3A%20Biological%20Oxidations%29 Open Library] or [https://archive.org/search?q=Methods%20in%20Enzymology%20Volume%20LIV%20Biomembranes%20%28Part%20E%3A%20Biological%20Oxidations%29 Internet Archive].

VOLUME LIII. Biomembranes (Part D: Biological Oxidations)

2026-07-06T01:18:07Z

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Available at [https://openlibrary.org/search?q=Methods%20in%20Enzymology%20Volume%20LIII%20Biomembranes%20%28Part%20D%3A%20Biological%20Oxidations%29 Open Library] or [https://archive.org/search?q=Methods%20in%20Enzymology%20Volume%20LIII%20Biomembranes%20%28Part%20D%3A%20Biological%20Oxidations%29 Internet Archive].