VOLUME 574. Enzymes of Epigenetics, Part B

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"In Volume 2, Chapters 1 through 8 cover Epigenetic Technologies. This includes identification and quantification of histone PTMs using high resolution mass spectrometry (Chapter 1), substrate specificity profiling of histone-modifying enzymes using peptide microarray (Chapter 2), an open-source platform to analyze such microarrays (Chapter 3), chemical biology approaches for characterization of epigenetic regulators (Chapter 4), mapping lysine acetyltransferase–ligand interactions by activity-based capture (Chapter 5), methods for investigating histone acetylation stoichiometry and turnover rate (Chapter 6), semisynthesis of acetylated and sumoylated histone analogs (Chapter 7), and an IF-FISH approach for covisualization of gene loci and nuclear architecture in fission yeast (Chapter 8). In Volume 2, Chapters 9 through 11 cover Small-Molecule Epigenetic Regulators and include the preparation and analysis of sirtuin deacetylase inhibitors (Chapter 9) and activators (Chapter 10) and methyltransferase inhibitors (Chapter 11). In Volume 2, Chapters 12 through 15 cover Epigenetics and Biological Connections and include exploring the dynamic relationship between cellular metabolism and chromatin structure using SILAC-mass spec and ChIP sequencing (Chapter 12), proteomic methods to investigate the dynamics of histone turnover in the central nervous system (Chapter 13), ChIP-seq techniques to map the epigenome of senescent cells (Chapter 14), and exploiting chromatin biology to understand immunology (Chapter 15)."